Research Paper Volume 9, Issue 12 pp 2559—2586

Central role of the p53 pathway in the noncoding-RNA response to oxidative stress

Figure 2. Differential MDM2 exon usage. (A) Genomic structure of the human MDM2 promoter region. The locus has two independent promoters before exons 1a (P1) and 1b (P2), yielding long- and short-5’ UTR isoforms, respectively. Translation start site (ATG) in exon 2 is indicated. Red triangles indicate p53 protein binding sites involved in P2 activation. (B) In H2O2 treated HUVEC, short-5’ UTR isoforms of MDM2 were induced, as assessed by qPCR using primers spanning from exon 1b to exon 2. Modulation of long-5’ UTR isoforms of MDM2 was not statistically significant, as assessed by qPCR using primers spanning from exon 1a to exon 2. The bar graph represents average values ±SEM (n= 3; *p<0.05, ***p<0.001).