Research Paper Volume 9, Issue 12 pp 2559—2586

Central role of the p53 pathway in the noncoding-RNA response to oxidative stress

Figure 4. miRNAs differentially expressed upon HUVEC exposure to H2O2. (A) HUVEC were exposed to H2O2 for 16 hrs and miRNA expression was measured by small RNA-sequencing (n= 3). Validation was performed by qPCR in independent HUVEC cultures treated with H2O2 for 16 hrs and 36 hrs (n= 3). The heat map shows modulated lncRNAs as log2 values. Green= down-modulation; red= up-regulation. (B) Interactions between qPCR-validated miRNAs and their potential targets, as reported by miRTarBase, that showed a significant down-regulation at both 16 hrs and 36 hrs time points in rRNA-depleted RNA-sequencing data. Results were represented using Cytoscape: For each gene, the inner color represents the log2 fold change at 16 hrs and the border color represents the log2 fold change at 36h. (C) miR-192-5p targets enrichment analysis. Enrichment analysis performed with ClueGO on miR-192-5p targets that showed a significant down regulation at both time points in rRNA-depleted RNA-sequencing data. Circles represent specific ontology terms or KEGG pathways that are significantly enriched. Edges represent terms connections within the ontology tree and colors highlight terms correlated in meaning. Terms are captioned if they are the most significant of the group or if they show a biological meaning connected to the system under analysis.