Research Paper Volume 9, Issue 12 pp 2559—2586

Central role of the p53 pathway in the noncoding-RNA response to oxidative stress

Figure 5. miR-192-5p overexpression inhibits EC growth. HUVEC were transfected with miR-192-5p mimic or control oligonucleotides. The next day, cells were plated at 200 000 cells/p3 plate density (T0) and harvested 24 and 48 hrs later. (A) The expression of the indicated genes was measured by qPCR at 24 hrs from T0. The bar graph represents average values ±SEM (n= 3; *p<0.05 **p<0.01). (B) Cells were counted at 24 hrs and 48 hrs from plating (T0) and plotted as % of the T0 cell number. miR192-5p expression inhibited HUVEC growth significantly. The graph represents average values ±SEM (n=8 independent transfections; **** p<0.0001).