Research Paper Volume 9, Issue 12 pp 2559—2586

Central role of the p53 pathway in the noncoding-RNA response to oxidative stress

Figure 6. p53 silencing inhibits the induction of target ncRNAs by H2O2. HUVEC were transfected with p53 or control (ctr) siRNAs and, 24 hrs later, treated with either 200 μM H2O2 or solvent alone. After 16 hrs, total RNA was extracted and the indicated RNAs analyzed by qPCR. (A) coding RNAs; (B) ncRNAs. The bar graphs show average fold changes ±SEM of the indicated RNAs referred to untreated cells transfected with control siRNA (n= 3; *p<0.05,**p<0.01, ***p<0.001).