Figure 7. ncRNA alterations identified in H2O2-treated ECs are also present in senescent HUVEC. Total RNA was extracted from early and late passage HUVEC and the indicated RNAs were tested by qPCR. The bar graphs represent average values ±SEM (early passage n= 3, late passage n= 7; *p<0.05, **p<0.01, ***p<0.001, ****p<0.0001). (A) ncRNAs. (B) Differential exon usage. Exon 1b isoforms of MDM2 and PVT1 are p53-regulated.