Research Paper Volume 9, Issue 12 pp 2587—2609

Mitogen-activated protein kinases, Fus3 and Kss1, regulate chronological lifespan in yeast

Figure 2. Cells lacking FUS3 or KSS1 exhibit elongated lifespan. (A) Qualitative CLS was assayed for the indicated yeast strains at days 3, 10, 17, and 24 growing in SD medium with 2% glucose. 10-fold serial dilutions of each CLS culture were spotted (5 μl) onto rich media (YPD) agar plates and grown for 48 hours at 30oC followed by image capture on a flatbed scanner. (B) Quantitative CLS (qCLS) measurements from cultures treated as in A. Percent survival was calculated based on the outgrowth curves obtained from 24hr growth of cultures in microtiter plates (see Materials and Methods). Error bars represent standard deviation across 3 analytical replicate experiments. For clarity, growth curves are separated to highlight fus3Δ (left); kss1Δ (middle); and hog1Δ (right) as well as controls for wild type lifespan (WT), increased (tor1Δ) and decreased (ach1Δ) lifespan.