Research Paper Volume 9, Issue 12 pp 2587—2609

Mitogen-activated protein kinases, Fus3 and Kss1, regulate chronological lifespan in yeast

Figure 5. Yeast lacking any one MAPK exhibit a decrease in CLS upon nitrogen starvation. (A-B) Qualitative CLS spot assay for the indicated genes grown in the presence (A) or absence (B) of ammonium sulfate. (C-G) qCLS curves comparing the survival decay of each mapkΔ strain and tor1Δ in the presence and absence of ammonium sulfate, representing nitrogen-optimal versus nitrogen-starved conditions, respectively.