Research Paper Volume 9, Issue 12 pp 2587—2609

Mitogen-activated protein kinases, Fus3 and Kss1, regulate chronological lifespan in yeast

Figure 6. FUS3 and TOR1 interact genetically to control CLS. (A-C) qCLS assay comparing the survival decay of mapkΔ/tor1Δ double deletion strains versus wild type and single gene deletion strains (overlaid from Figure 2, conducted on same plates). (D) Relative rates of survival decay from day5 to day17 were calculated using the slope value from linear regression analysis for each indicated strain. (E) The slope of the model (bars) and the coefficient of determination (r2) value representing the model fit (inset numbers) are shown to indicate the difference in relative decay rates of WT, single, and double gene deletion strains. Error bars throughout the figure represent the standard deviation across 3 analytical replicate experiments.