Figure 7. Cells expressing activation site mutant forms of Fus3 or Hog1 exhibit distinctive survival decay phenotypes. (A-C) qCLS assay comparing the survival decay of MAPK activation site mutants versus single MAPK gene deletions (overlaid from Figure 2, conducted on same plates). Error bars throughout the figure represent the standard deviation across 3 analytical replicate experiments. (D-F) Western blot analysis of MAPK protein levels in cells harvested from experiments A-C. Activation site mutants are indicated as follows: FAM, Fus3T180A,Y182F; KAM, Kss1T183A,Y185F; and HAM, Hog1T174A,Y176F. Notably, Kss1 is absent from quiescent fus3Δ but not FAM cells. (*) A non-specific band above Kss1 is common to western blots with this antibody.