Research Paper Volume 9, Issue 12 pp 2610—2628

Targeting flavin-containing enzymes eliminates cancer stem cells (CSCs), by inhibiting mitochondrial respiration: Vitamin B2 (Riboflavin) in cancer therapy

Figure 11. DPI rapidly induces the inhibition of mitochondrial respiration. Even with as little as 1 hour of DPI treatment, the mitochondrial oxygen consumption rate (OCR) was progressively reduced, over a concentration range of 10 to 100 nM, as seen using the Seahorse XFe96 Metabolic Flux Analyzer. Note that basal respiration was inhibited with an IC-50 of 50 nM.