Research Paper Volume 9, Issue 12 pp 2610—2628

Targeting flavin-containing enzymes eliminates cancer stem cells (CSCs), by inhibiting mitochondrial respiration: Vitamin B2 (Riboflavin) in cancer therapy

Figure 4. DPI potently inhibits mitochondrial respiration. After 24 hours of treatment with DPI (2.5 to 50 nM), MCF7 cells were subjected to metabolic flux analysis with the Seahorse XFe96, which measures the OCR (the oxygen consumption rate). Note that at concentration of 2.5 nM, little or no effect was observed. However, at 5 nM, basal respiration was reduced by ~ 50%. *** p<0.001.