Figure 5. DPI induces a reactive glycolytic response. After 24 hours of treatment with DPI (2.5 to 50 nM), MCF7 cells were subjected to metabolic flux analysis with the Seahorse XFe96, which also measures ECAR (the extracellular acidification rate), a surrogate marker for L-lactate production. Note that at a concentration of 2.5 nM, little or no effect was observed. However, at 10 nM, glycolysis was increased by 2-fold. *** p<0.001.