Research Paper Volume 9, Issue 12 pp 2610—2628

Targeting flavin-containing enzymes eliminates cancer stem cells (CSCs), by inhibiting mitochondrial respiration: Vitamin B2 (Riboflavin) in cancer therapy

Figure 5. DPI induces a reactive glycolytic response. After 24 hours of treatment with DPI (2.5 to 50 nM), MCF7 cells were subjected to metabolic flux analysis with the Seahorse XFe96, which also measures ECAR (the extracellular acidification rate), a surrogate marker for L-lactate production. Note that at a concentration of 2.5 nM, little or no effect was observed. However, at 10 nM, glycolysis was increased by 2-fold. *** p<0.001.