Research Paper Volume 9, Issue 12 pp 2610—2628

Targeting flavin-containing enzymes eliminates cancer stem cells (CSCs), by inhibiting mitochondrial respiration: Vitamin B2 (Riboflavin) in cancer therapy

Figure 6. DPI drives the production of L-lactate. After treatment with DPI (5 or 50 nM) for 1, 3 or 5 days, the cell culture media from MCF7 cells was subjected to analysis using the ISCUS-flex microdialysis analyser, to directly measure L-lactate content. Note that DPI induces significant L-lactate production, nearly doubling the amount of lactate as early as 1 day of treatment, using only 5 nM DPI. *** p<0.001.