Research Paper Volume 9, Issue 12 pp 2610—2628
Targeting flavin-containing enzymes eliminates cancer stem cells (CSCs), by inhibiting mitochondrial respiration: Vitamin B2 (Riboflavin) in cancer therapy
Figure 7. Dose-dependent inhibition of CSC propagation using DPI, as measured using the mammosphere assay. MCF7 cells were seeded into low-attachment plates and allowed to form mammospheres (a.k.a., 3D tumor spheres), over a period of 5 days. Note that DPI markedly reduced CSC propagation, with an IC-50 of 3.23 nM. The mammosphere assay was performed over the range of 0.2 nM to 10 µM.