Research Paper Volume 9, Issue 12 pp 2610—2628

Targeting flavin-containing enzymes eliminates cancer stem cells (CSCs), by inhibiting mitochondrial respiration: Vitamin B2 (Riboflavin) in cancer therapy

Figure 9. Effect of DPI treatment on mitochondrial ROS production.The effects of DPI on mitochondrial ROS production were determined over the range of 5 to 50 nM. Note that at a concentration of 5 nM, DPI failed to induce mitochondrial ROS production. In contrast, 50 nM DPI induced the same amount of mitochondrial ROS as 500 nM Rotenone, which was used as a positive control. Mitochondrial ROS production was monitored by FACS analysis, using MitoSOX as a fluorescent indicator. * p<0.05.