Research Paper Volume 10, Issue 1 pp 56—71

NKILA inhibits NF-κB signaling and suppresses tumor metastasis

Figure 5. Enforced NKILA expression inhibits proliferation and metastasis in ESCC cells. (A) Overexpression of NKILA in KYSE30 and KYSE520 cells was confirmed by qPCR assays. (B) CCK-8 assays of KYSE30 and KYSE520 cells after overexpression of NKILA. (C) Colony formation assays of KYSE30 and KYSE520 cells after overexpression of NKILA. Upper panel was representative images and lower panel was statistical quantification. (D) Migration assays of KYSE30 and KYSE520 cells after overexpression of NKILA. Left panel was representative images and right panel was statistical quantification. (E) Tumor growth curve of KYSE30/EV and KYSE30/NKILA cells. The dissected tumors from the nude mice was photographed. (F) Weight of dissected tumors was recorded. (G) Immunohistochemical analysis of Ki-67 in the dissected tumors. Left panel was representative images and right panel was statistical quantification. (H) Expression level of NKILA in the dissected tumors was detected by qPCR. (I) Representative H&E staining of lung tissue sections formed by KYSE30/EV and KYSE30/NKILA cells. Scale bars: 50μm. The micro-metastasis in the lung was numbered. Data in A, B, C and D represents the mean ± SD of three repeated experiments. Data in E, F, G, H and I represents the mean ± SD of six mice. *P < 0.05.