Figure 1. Proliferation of rabbit AFSCs cultured in growth medium with five different conditions for 6 days. (A, F) Growth medium only (Control); (B, G) bleomycin 10 μg/ml (B-10); (C, H) bleomycin 50 μg/ml (B-50); (D, I) Bleomycin 50 μg/ml and rapamycin 25 nM (B-50 + R-25); (E, J) Bleomycin 50 μg/ml and rapamycin 50 nM (B-50 + R-50); (L) Population doubling time (PDT) of AFSCs grown in five different conditions for 6 days. The images of F, G, H, I, J were enlarged areas of the boxes in images of A, B, C, D, E. Bleomycin treatment changed the morphology of AFSCs from cobblestone-like cells (green arrows in A, F) to pancake-like cells (red arrows in B, C, G, H), and also decreased proliferation of AFSCs as evidenced by population doubling time (L). However, the addition of rapamycin into bleomycin-containing medium decreased pancake-like cell numbers and increased spindle-like cell numbers (yellow arrows in D, E, I, J). White bars: 100 μm, yellow bars: 50 μm. *p<0.05 compared to control.