Figure 2. Senescence of rabbit AFSCs cultured in growth medium with five different conditions for 6 days and stained by histochemical staining kit. (A, F) Growth medium only (Control); (B, G) bleomycin 10 μg/ml (Bleo-10); (C, H) bleomycin 50 μg/ml (Bleo-50); (D, I) Bleomycin 50 μg/ml and rapamycin 25 nM (Bleo-50 + Rapa-25); (E, J) Bleomycin 50 μg/ml and rapamycin 50 nM (Bleo-50 + Rapa-50); (L) Semi-quantification of positive stained AF cells grown in five different conditions for 6 days. The images of F, G, H, I, J were enlarged areas of the boxes in images of A, B, C, D, E. The results indicated that bleomycin treatment induced the senescence of AF cells which was stained by green. Rapamycin decreased cell senescence induced by bleomycin. White bars: 100 μm, Black bars: 50 μm. *p<0.05 compared to control, #p<0.05 compared to the cells treated with 50 μg/ml of bleomycin.