Research Paper Volume 10, Issue 1 pp 131—143

Rapamycin prevents the intervertebral disc degeneration via inhibiting differentiation and senescence of annulus fibrosus cells

Figure 8. Rapamycin inhibited differentiation of rabbit AFSCs in a concentration-dependent manner (*P < 0.05). The AFSCs were cultured in various differentiation media with rapamycin (0, 25, 50 nM) for 21 days. The adipocytes were stained by Oil Red O (A-C, J), osteocytes were stained by Alizarin Red S (D-F, J), and chondrocytes were stained by Safranin O (G-I, J), respectively. The differentiation potentials of AFSCs cultured with various conditions were analyzed by semi-quantification shown in (J).