Research Paper Volume 10, Issue 3 pp 322—338

Deficiency in DNA damage response of enterocytes accelerates intestinal stem cell aging in Drosophila

Figure 3A. EC-specific knockdown of DNA damage response (DDR)-related factors causes an increase in the age-related phenotypes of ISCs. EC-specific knockdown of Mre11, Rad50, Nbs1, ATM, ATR, Chk1, or Chk2 induce DNA damage accumulation in ISCs. Flies carrying Myots>GFP, Myots>GFP+Mre11i, Myots>GFP+Rad50i, Myots>GFP+Nbs1i, Myots>GFP+ATMi, Myots>GFP+ATRi, Myots>GFP+Chk1i, or Myots>GFP+Chk2i genotypes were cultured at 29 °C for 4 days. The guts of flies were dissected and labeled with anti-GFP (green), anti-Delta (white), and anti-γH2AvD (red) antibodies and DAPI (blue). Yellow arrow heads indicate Delta+ cell. Upper two panels is merged image. Lower four panels is gray scale image of upper images. Asterisk indicates Myo-, Delta-, and strong γH2AvD+ cell, shows dying cell.