Research Paper Volume 10, Issue 3 pp 402—424

Preferential Ty1 retromobility in mother cells and nonquiescent stationary phase cells is associated with increased concentrations of total Gag or processed Gag and is inhibited by exposure to a high concentration of calcium

Figure 5. Ty1 retromobility asymmetry between quiescent and nonquiescent stationary phase cells also depends on LSM1, calcium, and pH homeostasis. (A) His+ frequencies in stationary phase cells grown in YPD and fractionated into quiescent (Q) and nonquiescent (NQ) subpopulations for the indicated genotypes or with chronic exposure to 100 mM calcium chloride. Results from three to six trials are shown. (B) Final (stationary phase) medium pH for Q and NQ cells from wild type or brp1∆ mutants for cells grown in control YPD medium, or YPD medium with an initial pH of 7.1 or 4.1 due to addition of 20 mM sodium phosphate (Na-phos) or 30 mM hydrochloric acid (HCl), respectively. Data are from three to five trials each. (C) His+ frequencies in Q and NQ cells for the strains and control or alternative media conditions from panel B. Control wild type data are the same as in panel A. (D) His+ frequencies in Q and NQ cells fractionated from the indicated strains after growth in SC medium. Results are for three to five trials. (E) The proportion of Q cells after fractionation of cells grown to stationary phase in YPD or YP + 2% ethanol (YPE) for three trials. (F) His+ frequencies in Q and NQ cells isolated after growth in YPD or YPE for three trials. All graphs show mean values with standard deviation, and symbols for statistical significance are as for Fig. 2.