Research Paper Volume 10, Issue 3 pp 434—462

Induction, regulation and roles of neural adhesion molecule L1CAM in cellular senescence

Figure 7. L1CAM levels correlate with enhanced cell migration and adhesion both in proliferating and senescent cells. (A) Wound healing assay of proliferating BJ after RNA interference-mediated knockdown of L1CAM and (B) replicatively senescent BJ fibroblasts sorted according to cell surface expression of L1CAM. (C) 3D migration assay of replicatively senescent BJ sorted according to L1CAM cell surface expression presented as velocity (left chart), Euclidian distance (middle chart) and accumulated distance (right chart). (D) 3D migration assay of unsorted replicatively senescent BJ. Left chart – velocity; middle chart – Euclidian distance; and right chart – accumulated distance. (E) Adhesion assay of replicatively senescent BJ fibroblasts sorted according to cell surface L1CAM expression and (F) after L1CAM knockdown. All experiments were performed in three independent replicates. p ˂ 0.05 (*); p ˂ 0.01 (**); p ˂ 0.001 (***), two-tailed Student’s t-test.