Figure 1. qRT-PCR analysis of nicotinic acetylcholine receptor (nAChRs) subunit mRNA and immunolocalization of MT1 protein in the neonatal mouse ovaries. (A) Relative changed of nAChR in mRNA expression in the normal control, intraperitoneally injected 1 mg/kg nicotine treated mouse ovaries. The results are presented as mean±SD. All the experiments were repeated at least three times. * P < 0.05; ** P < 0.01. (B) Immunolocalization of MT1 receptor in neonatal mouse ovarian follicles (arrows) and negative control.