Research Paper Volume 10, Issue 5 pp 951—972

Non-senescent Hydra tolerates severe disturbances in the nuclear lamina

Figure 2. Lamin protein from Hydra is recruited to the nuclear envelope of mammalian cells, if it contains an intact CaaX-box. (A) Maps of two vectors used for the heterologous expression of Hydra HyLMN in COS-7 cells. (B) Transfection of COS-7 cells with a pCMV-Myc-Vector containing full CDS of the hyLMN results in the expression of Myc-tagged HyLMN protein (Myc-HyLMN) localized to the nuclear envelope. If a stop-codon is introduced into the hyLMN CDS resulting in the truncated HyLMN protein lacking the C-terminal CaaX-box (Myc-HyLMNΔCaaX), the protein is not integrated anymore into the nuclear envelope and accumulates in the nucleoplasm.