Research Paper Volume 10, Issue 5 pp 988—999

A novel functional polymorphism of GFAP decrease glioblastoma susceptibility through inhibiting the binding of miR-139

Figure 3. miR-139 inhibited GFAP expression in U251 cells. (A, B) Western blot analysis for GFAP expression in GBM tissues from patients with different genotypes (A) and corresponding quantitative analysis (B). (C, D) Western blot assay of GFAP in U251 cells transfected with miR-139 mimic or antagomir-miR-139 in different doses. (E, F) Gray scale quantification of the GFAP protein expression levels. Each experiment was repeated three times. *P<0.05, **P<0.01, ns: non-significant compared between treatment group and control group.