Research Paper Volume 10, Issue 5 pp 1089—1102

Repeated superovulation increases the risk of osteoporosis and cardiovascular diseases by accelerating ovarian aging in mice

Figure 5. The evaluation of ovarian endocrine and reproductive function. (A) The ovarian weight of the RS group mice increased significantly compared with the NC group mice. (B) The estrous cycles of the RS mice were obviously prolonged. The concentrations of estradiol (C) and progesterone (D) were determined in duplicate using an EIA kit. The mean litter size (E) and the mean number of litters (F) were analyzed during a 6-month course of breeding. The RS mice displayed lower levels of hormones and reduced litter sizes and numbers compared to the NC group. Data presented are the mean ± SEM. * P<0.05 compared to the NC.