Research Paper Volume 10, Issue 5 pp 1133—1145

Chromatin changes trigger laminin genes dysregulation in aging kidneys

Figure 3. Western blot analysis of H3K27m3 levels in histones extracted from young and old kidneys. Upper panel, equal amounts of acid-extracted proteins from nuclear-enriched tissue fraction were subjected to SDS PAGE in 13% gels, transferred to PVDF membranes and stained with anti-H3K27m3 antibodies. Representative parts of ponseau-stained membranes are also shown (Load). Lower panel, results of densitometry analysis of bands shown in the upper panel. Y, young (4mo), O, old (28mo for F344, 32mo for FBN-F1). Signal intensities were normalized to load. Shown are mean values +SD, **p<0.005.