Figure 5. Immunolocalization of p53 during spermatogenesis in E. sinensis. Red: p53, Green: Tubulin, Blue: Nucleus. N: nucleus, PG: proacrosomal granule, PV: proacrosomal vesicle, AT: acrosome tube, AC: acrosome cap, AV: acrosome vesicle, FL: fibrous layer, ML: middle layer, LS: lamellar structure, MC: membrane complex, RA: radical arm, NC: nuclear cap, MT: mitochondria. (A1-A4) spermatogonia, (B1-B4) spermatocyte, (C1-C4) stage I spermatid, (D1-D4) stage II spermatid, (E1-E4) stage III spermatid, (F1-F4) mature sperm. The intensity of p53 in cytoplasm are enhanced from spermatogonia to spermatocyte. In stage II spermatids, signals are found in the proacrosome and partial cytoplasm. In the mature sperm, p53 are found around the rim of the cup-shaped nucleus. Bars=20 um.