Figure 2. Mitochondrial fuel usage is altered during doxorubicin-induced senescence. (A) Glucose uptake rate was measured by 2-NBDG, a fluorescently labeled deoxyglucose analog. Quantification and representative western blots of (B) carnitine palmitoyltransferase I (CPT1A) and (C) glutaminase (GLS1), both showing beta-actin as a loading control. Data are reported as mean ± SEM of three to six independent experiments. Significant differences were determined by Student’s t-tests. *p<0.05. Abbreviations: NS, Non-senescent cells (quiescent); SEN, Senescent cells.