Research Paper Volume 10, Issue 6 pp 1257—1267

Abnormal gut microbiota composition contributes to cognitive dysfunction in SAMP8 mice

Figure 1. MWMT for SAMR1 and SAMP8 mice. (A) The schedule for MWMT and fecal sample collection. Seven days after accommodation, SAMR1 and SAMP8 mice were scheduled for MWMT and probe trial on days 1–6. On day 7, fecal samples were collected for 16S rRNA gene sequencing and fecal bacteria transplant. (B) Body weight (T-test, P > 0.05). (C) The trace graph of SAMR1 and SAMP8 mice in MWMT. (D) Escape latency (two-way ANOVA; Time: F4,20 = 6.062, P < 0.01; Group: F1,5 = 8.802, P < 0.05; Interaction: F4,20 = 3.476, P < 0.05). (E) Escape path length (two-way ANOVA. Time: F4,20 = 11.32, P < 0.001; Group: F1,5 = 4.834, P = 0.0792; Interaction: F4,20 = 3.271, P < 0.05). (F) Platform crossing (t-test, P < 0.05). (G) Time spent in each quadrant (t-test, P < 0.05). ANOVA: analysis of variance; MWMT: Morris water maze test. Data are shown as mean ± SEM (n = 6). *P < 0.05, **P < 0.01.