Figure 2. RAB3D is a direct target of miR-506-3p in osteosarcoma. (A) miRDB predicted miR-506-3p specifically combines with RAB3D. (B) Expression of RAB3D mRNA in osteosarcoma and adjacent normal tissues detected using real-time PCR. Data are shown as the mean ± SEM. ** P< 0.05 vs. adjacent tissues. (C) Relationship between RAB3D and survival in osteosarcoma patients. (D) Expressions of RAB3D in hFOB 1.19, HOS, U2OS, and MG63 cells detected using real time PCR. ** P< 0.05 vs. hFOB 1.19 cells. (E) Correlation between expression levels of miR-506-3p and RAB3D in osteosarcoma. (F) Interaction between miR-506-3p and the RAB3D 3’-UTR tested in luciferase reporter assays. Data are shown as the mean ± SEM. **P< 0.05 vs. control. (G-J) Western blot and real time PCR analyses showing that when miR-506-3p is overexpression/repressed the expression of RAB3D was down/upregulated. Data are shown as mean ± SEM. ** P< 0.05 vs. control.