Figure 4. MiR-506-3p inhibited metastasis of osteosarcoma cells. (A-D) Transwell assays with and without Matrigel showing the effect of miR-506-3p expression/repression on the migration and invasiveness of HOS and MG63 cells. Cells were counted and results are the mean ± SD of three experiments. ** P< 0.05. (E-H) Western blot and real-time PCR analysis of MMP2 expression in osteosarcoma cells transfected with miR-506-3p mimic/inhibitor. Data are shown as the mean ± SEM. ** P< 0.05.