Research Paper Volume 10, Issue 6 pp 1306—1323

Loss of NAMPT in aging retinal pigment epithelium reduces NAD+ availability and promotes cellular senescence

Figure 7. Time-dependent changes in SIRT-1 expression/activity and inflammatory markers in human retinal pigment epithelial cells treated with FK866. Human retinal pigment epithelial cells (ARPE-19) were treated with 10μM FK866 for 24, 48 and 72 hr. and (A) expression and (B) activity of SIRT-1 was evaluated by western blotting, and commercially available SIRT-1 assay kit respectively. (C-D) Changes in inflammatory markers (IL-6 and IL-8) were evaluated by qPCR. (E) Representative images of CellROX and MitoSOX stained 10μM FK866 treated (72 hr.) A representative western blot image from three replicates is shown. mRNA expression of genes were normalized to 18s expression. Data are presented as mean ± S.E.M for n=3 independent experiments. *p<0.05 compared to CON (Vehicle treated).