Research Paper Volume 10, Issue 6 pp 1380—1389

The expression of C1 inhibitor (C1INH) in macrophages is upregulated by retinal pigment epithelial cells – implication in subretinal immune privilege in the aging eye

Figure 2. The effects of normal RPE cell on BMDM complement expression. BMDMs from C57BL/6J mice were co-cultured with primary mouse RPE cells for 7h (A) or 24h (B). Macrophages were then isolated by CD11b+ MACS kit and processed for real-time RT-PCR analysis of complement genes (A) and western blot analysis of C1INH protein expression (B). Fold change of C1INH protein expression by BMDMs after co-culture was analyzed by ImageJ (C). Mean ± SEM, n =3; *, P< 0.05; **, P < 0.01 compared to naïve BMDM alone, Unpaired Student t test.