Figure 3. The effects of oxidized POS treated-RPE cell on BMDM complement gene expression. RPE cells were treated with oxidized photoreceptor outer segments (oxPOS) for 24h. oxPOS were then removed from the culture. (A) β-galactosidase expression in ox-POS-treated RPE cells. (B) The oxPOS pre-treated RPE cells were co-cultured with naïve BMDMs for 7h. Macrophages were isolated and processed for real-time RT-PCR analysis of complement genes. Mean ± SEM, n =3; *, P< 0.05; **, P < 0.01 compared to naïve BMDM alone, Unpaired Student t test.