Figure 4. The effects of TNF-α treated-RPE cell on BMDM complement gene expression. RPE cells were treated with TNF-α for 16h. Naïve BMDMs were then co-cultured with TNF-α pre-treated RPE cells for 7h. Macrophages were isolated and processed for real-time RT-PCR analysis of complement genes. Mean ± SEM, n =3; *, P< 0.05; **, P < 0.01 compared to naïve BMDM alone, Unpaired Student t test.