Figure 5. The effects of apoptotic RPE cell on BMDM complement gene expression. Primary mouse RPE cells were treated with H2O2 in serum-free DMEM overnight. Apoptotic RPE cells were confirmed by Propidium iodide (PI) and Annexin V staining (A). The apoptotic RPE cells were then incubated with naïve BMDMs for 7h. Macrophages were isolated and processed for real-time RT-PCR analysis of complement genes (B). Mean ± SEM, n =3; *, P< 0.05; **, P < 0.01, ***, P < 0.001 compared to naïve BMDM alone, Unpaired Student t test.