Research Paper Volume 10, Issue 6 pp 1380—1389

The expression of C1 inhibitor (C1INH) in macrophages is upregulated by retinal pigment epithelial cells – implication in subretinal immune privilege in the aging eye

Figure 5. The effects of apoptotic RPE cell on BMDM complement gene expression. Primary mouse RPE cells were treated with H2O2 in serum-free DMEM overnight. Apoptotic RPE cells were confirmed by Propidium iodide (PI) and Annexin V staining (A). The apoptotic RPE cells were then incubated with naïve BMDMs for 7h. Macrophages were isolated and processed for real-time RT-PCR analysis of complement genes (B). Mean ± SEM, n =3; *, P< 0.05; **, P < 0.01, ***, P < 0.001 compared to naïve BMDM alone, Unpaired Student t test.