Research Paper Volume 10, Issue 6 pp 1390—1401

Five ETS family members, ELF-1, ETV-4, ETV-3L, ETS-1, and ETS-2 upregulate human leukocyte-associated immunoglobulin-like receptor-1 gene basic promoter activity

Figure 4. Effect of ELF-1, ETV-4, ETV-3L, ETS-1 and ETS-2 on LAIR-1 gene expression in HO8910 cells. HO8910 cells were transfected with pGL-lair1-p6 (-256/-8) and expression vector of ELF-1 (pCDB-ELF1), ETV-3L (pCDB-ETV3L), ETV-4 (pCDB-ETV4), ETS-1 (pcDNA-ETS1) or ETS-2 (pSPORT-ETS2). At 48 h post-transfection, cells were harvested. (A) Total RNA was used to detect the LAIR-1 mRNA level through qRT-PCR. The relative mRNA level was obtained after comparison with the empty vector, which was set to 1. (B) LAIR-1 protein expression was analyzed by Western blotting. The experiments were repeated at least thrice, and the data from one representative experiment with two technical repeats were presented (**, p < 0.01; ***, p < 0.001).