Figure 2. Secreted α-Klotho expression during in vitro chondrogenesis. TGFβ3-driven chondrogenic differentiation of BM-MSCs (osteochondral progenitors) in micropellet culture (n=3). Expression analysis of secreted α-KL (A) by RT-qPCR (gene expression level relative to that of RPS9) at different time points during BM-MSC differentiation into chondrocytes. (B) α-Klotho protein expression detectable below 65kDa marker by western blotting in conditioned medium from BM-MSCs in micromass culture at the different time points during chondrogenesis. Data are represented as mean -/+ SEM.