Figure 6. Astrogliosis could be regulated though intervention of PI3K/Akt/mTOR signaling. Western blot and immunofluorescence two groups: one transfected with miR-21 OE or NC with or without LY294002; and a second treated with TGF-β1 or PBS with or without LY294002. (A) Bax, Bcl2, PCNA, caspase 3, and GAPDH were evaluated (B–G) and analyzed by ImageJ and SPSS. (H and I) Immunofluorescence was used for the detection of Ki-67 (H). (J-K) The results were analyzed by ImageJ and SPSS. Data are expressed as mean ± SD. *P < 0.05, **P < 0.01, ***P < 0.001. Akt, protein kinase B; Bcl2, B-cell lymphoma 2; GAPDH, glyceraldehyde 3-phosphate dehydrogenase; GFAP, glial fibrillary acidic protein; mTOR, mechanistic target of rapamycin; PCNA, proliferating cell nuclear antigen; PI3K, phosphoinositide 3-kinase; SD, standard deviation.