Figure 2. Linc00472 overexpression inhibited proliferation and induced apoptosis in CRC cells. HT-29 and HCT-116 cells were transfected with pcDNA3.1 vector (Vector) or pcDNA-Linc00472 (Linc00472). (A) The Linc00472 expression level was detected using qRT-PCR assays. (B and C) Cell proliferation was measured with CCK-8 assays. (D and E) Cell clone numbers were evaluated by colony formation assay. (F and G) Cell apoptosis was determined by flow cytometry analysis. *P < 0.05.