Figure 3. Linc00472 directly inhibited miR-1496a expression. (A) The predicted binding sites between Linc00472 and miR-141 were shown. (B) The luciferase activity was detected in 293T cells. (C) qRT-PCR analysis miR-196a expression in HT-29 and HCT-116 cells transfected with pcDNA3.1 vector (Vector) or pcDNA-Linc00472 (Linc00472). (D) qRT-PCR assay was performed to detect miR-196a expression in CRC tumor tissues and adjacent normal tissues. (E) Linc00472 expression was analyzed in TCGA COAD cohort. (F) Correlation analysis between Linc00472 and miR-196a in CRC tissues from TCGA COAD dataset. *P < 0.05, ***P < 0.001.