Figure 4. PDCD4 was a target of miR-196a. (A) The putative binding sites between PDCD4 and miR-196a by miRanda software. (B) The luciferase activity of wide-type or mutant-type PDCD4 was determined in 293T cells. (C) PDCD4 protein level in HT-29 and HCT-116 cells was detected using western blot analysis. (D) qRT-PCR assay analyzed PDCD4 mRNA levels in CRC tumor tissues and adjacent normal tissues. (E) PDCD4 mRNA levels were analyzed in TCGA COAD cohort. (F) Correlation analysis between Linc00472 and PDCD4 in CRC tissues from TCGA COAD dataset. *P < 0.05, ***P < 0.001.