Research Paper Volume 10, Issue 6 pp 1523—1533

Linc00472 suppresses proliferation and promotes apoptosis through elevating PDCD4 expression by sponging miR-196a in colorectal cancer

Figure 5. Linc00472 inhibited proliferation and enhanced apoptosis by modulating miR-196a/PDCD4 axis in CRC cells. HT-29 cells were transfected with Vector+miR-con, Linc00472+miR-con, Vector+miR-196a or Linc00472+miR-196a, and HCT-116 cells were transfected with Vector+si-con, Linc00472+si-con, Vector+si-PDCD4 or Linc00472+si-PDCD4. The PDCD4 protein levels were measured by western blot assays (A and B), the cell proliferation was evaluated by CCK-8 assays (C and D), the cell clone numbers were estimated by colony formation assays (E and F), and the cell apoptosis was determined with flow cytometry (G and H). *P < 0.05.