Research Paper Volume 10, Issue 8 pp 1884—1901

Anillin is required for tumor growth and regulated by miR-15a/miR-16-1 in HBV-related hepatocellular carcinoma

Figure 4. ANLN expression is required for HCC cell growth. (A) Representative images of colony formation assays of SMMC-7721 and QGY-7703 cells after ANLN depletion. (B) Quantification of the colony numbers in (A). (C) Representative images of soft agar assays of SMMC-7721 and QGY-7703 cells after ANLN depletion. (D) Quantification of the colony numbers in (C). (E) Western blotting analysis of the expression levels of cleaved caspase-3, cleaved PARP and cleaved caspase-9 in ANLN knockdown and control cells derived from plate and soft agar colonies. (F) Representative images of the sphere formation assay of SMMC-7721 and QGY-7703 cells after ANLN depletion. (G) Quantification of the spherical colony numbers in (f). ***P < 0.001.