Research Paper Volume 10, Issue 8 pp 1884—1901

Anillin is required for tumor growth and regulated by miR-15a/miR-16-1 in HBV-related hepatocellular carcinoma

Figure 5. ANLN depletion leads to poly-nucleated morphology and DNA damage in HCC cells. (A) Representative images of the immunofluorescence analysis of ANLN depletion and reconstitution in SMMC-7721 cells. DAPI stains the nucleus with blue fluorescence and GFP indicates the cell shape with green fluorescence. ANLN is shown in red fluorescence. Scale bar: 50 μm. (B) Quantification of the percentages of multinucleated cells in (A). (C) Representative images of immunofluorescence analysis of ANLN depletion and reconstitution in QGY-7703 cells. (D) Quantification of the percentages of multinucleated cells in (C). (E) Western blotting analysis of the expression levels of phosphorylated ATM (Ser1981), phosphorylated ATR (Ser428), phosphorylated Chk1 (Ser345), phosphorylated Chk2 (Thr68) and phosphorylated Histone H2A.X (Ser139) in ANLN knockdown and control cells.