Figure 1. Aging aggravates hepatic I/R injury in C57BL/6 mice. Young (Y) and old mice (O) were subjected to middle and left hepatic pedicle occlusion for 1 h before reperfusion (I-R) or sham operation (Sham). Liver and blood were collected 1 h after reperfusion. (A) Representative histology of liver by H&E staining and pathological score of liver damage. (B) Blood samples were collected for measurements of serum AST and ALT. (C) Reactive oxygen species generated by mitochondria was detected by MitoSOX Red staining. (D) Whole tissue lysate of liver was collected for immunoblot analysis of cleaved PARP (CL PARP). (E) Representative images of TUNEL staining of liver tissues and quantification of TUNEL-positive cells rate in liver tissues. The data are expressed as mean ± SD. Statistical comparisons were performed with t-test. *P < 0.05 vs. the indicated group. Scale bar: 50μm.