Figure 2. Mitophagy declines with age during hepatic I/R. Mice of different age were treated as indicated (Y for young mice, O for old mice, Y+CQ for young mice with chloroquine pretreatment, O+CQ for old mice with chloroquine pretreatment). (A) Representative images of LC3B staining of liver tissues by fluorescence microscopy. (B) Representative images of LC3B and TOMM20 staining of liver tissues by immunohistochemistry. (C) Representative TEM images of autophagosomes in hepatocytes and quantification of autophagosomes in hepatocytes. (D) The LC3B and TOMM20 protein levels were determined by western blot analysis from the indicated groups. The data are expressed as mean ± SD. Statistical comparisons were performed with t-test. *P < 0.05 vs. the indicated group, NS no significant difference. Scale bar: 50μm.