Research Paper Volume 10, Issue 8 pp 1902—1920

Aging aggravates hepatic ischemia-reperfusion injury in mice by impairing mitophagy with the involvement of the EIF2α-parkin pathway

Figure 3. Mitophagy protects liver from I/R injury. Young mice with CQ pretreatment (Y+CQ) or without (Y) were treated as indicated. (A) Representative histology of liver by H&E staining (top panel) and pathological score of liver damage from indicated groups; TUNEL staining (bottom panel) and quantification of TUNEL-positive cells rate from indicated groups. (B) The cleaved PARP (CL PARP) protein levels were determined by western blot analysis from indicated groups. (C) Measurements of serum AST and ALT from indicated groups. Old mice with Rapamycin pretreatment (O+RA) or without (O) were treated as indicated. (D) Representative histology of liver by H&E staining (top panel) and pathological score of liver damage from indicated groups; TUNEL staining (bottom panel) and quantification of TUNEL-positive cells rate from indicated groups. (E) The cleaved PARP (CL PARP) and TOMM20 protein levels were determined by western blot analysis from indicated groups. (F) Measurements of serum AST and ALT from indicated groups. The data are expressed as mean ± SD. Statistical comparisons were performed with t-test. *P < 0.05 vs. the indicated group. Scale bar: 50μm.