Figure 4. Parkin and Atg5 reduced in livers of old C57BL/6 mice during hepatic I/R. (A) The Atg5, Parkin, PINK1, Atg7, BECLIN1 and Atg3 protein levels were determined by western blot analysis from the liver tissue of young (Y) and old (O) mice. Representative images of Atg5 (B) and Parkin (C) staining of liver tissues by immunohistochemistry. The data are expressed as mean ± SD. Statistical comparisons were performed with t-test. *P < 0.05 vs. the indicated group. Scale bar: 50μm.